Towards Improved Accuracy of Bordetella pertussis Nucleic Acid Amplification Tests
نویسندگان
چکیده
منابع مشابه
Towards improved accuracy of Bordetella pertussis nucleic acid amplification tests.
In many clinical microbiology laboratories, nucleic acid amplification tests such as PCR have become the routine methods for the diagnosis of pertussis. While PCR has greatly increased the ability of laboratories to detect Bordetella pertussis infections, it has also been associated with false-positive results that can, given the tendency of B. pertussis to cause outbreaks, result in unnecessar...
متن کاملNucleic Acid amplification tests for diagnosis of Bordetella infections.
The global epidemiology of pertussis has recently been reviewed (12, 15). Bordetella pertussis continues to circulate even in populations where high vaccination coverage of infants and children is achieved (15, 23), because the protection after natural infection wanes after 10 to 15 years and protection after vaccination lasts for 6 to 10 years (15). A significant increase of pertussis cases wa...
متن کاملThe accuracy and reliability of nucleic acid amplification tests in the diagnosis of tuberculosis.
Accurate and early diagnosis of tuberculosis is a critical part of the management and control of the disease. Diagnostic work up for tuberculosis involves the detection of acid-fast bacilli in clinical samples by microscopy (smear) and culture. These conventional tests are not always helpful in making the diagnosis. Microscopy, although rapid and inexpensive, has only modest sensitivity and spe...
متن کاملNucleic acid amplification tests for the diagnosis of pneumonia.
Molecular diagnostic techniques, such as polymerase chain reaction (PCR), are promising tools for the rapid etiological diagnosis of pneumonia. PCR offers potential advantages over conventional tests for the detection of Mycoplasma pneumoniae, Legionella species, and Chlamydia pneumoniae. For pneumococcal pneumonia in adults, PCR adds little to existing diagnostic tests and is unable to disting...
متن کاملModulation of Bordetella pertussis by nicotinic acid.
Growth of Bordetella pertussis in a high concentration of nicotinic acid (NA) had a modulating effect on several properties and activities of the bacteria. Compared with normally grown cells, those grown in a high concentration of NA had reduced capacity for taking up both NA and nicotinamide (ND); they had reduced adenylate cyclase activity and showed loss of agglutinogen factors 2 and 3, but ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2012
ISSN: 0095-1137
DOI: 10.1128/jcm.00612-12